BD FACSAria Cell Sorting System
- High-speed sorter with fixed-alignment cuvette flow cell for superior
fluorescence sensitivity
- Three air-cooled lasers at 488-nm, 633-nm, and 407-nm wavelengths
- Digital acquisition rates of up to 70,000 events/second
- Multicolor analysis to 15 parameters
- Two- and four-way bulk sorting devices for a variety of tube sizes
- BDTM Automated Cell Deposition Unit (ACDU) for sorting to BD MultiwellTM
plates or microscope slides
- BDTM Aerosol Management Option
Barbara Walter
Flow Cytometer Technician
Keen Hall Room 114
827-7928
Barbara.Walter@ucr.edu |
| Uses
for Flow Cytometry |
| Category
of Study |
Parameters
monitored |
| Tests for Aneuploidy |
DNA content |
| Cell Cycle regulation |
DNA content, DNA/RNA ratios, cell cycle-specific target monitoring
with labeled probes |
| Cell Division/Generation number |
Generation-dependent loss of fluorescence from trapped dyes |
| DNA synthesis |
Fluorescence monitoring of immune- labeled BrdU or alternative
nucleotides |
| Apoptosis |
DNA content, mitochondrial membrane potential, caspase-substrate/inhibitor
interactions |
| Transformation |
Telomere length with use of PNA probes |
| Gene Expression |
Accumulation of reporter gene products, e.g., GFP, LacZ |
| Protein expression/degradation |
Surface and intracellular antigens: Receptor, cytokine, kinase
regulation |
|
Membrane
Dynamics |
Fluorescence monitoring of state-specific integrity, permeability
or organization via dye uptake (e.g., propidium iodide), surface-labeling
of antigens |
|
Signal
Transduction |
Second messenger, including calcium, concentration monitoring
with fluorescent dyes |
|
|